Yes, set the background color and the transparant color to the same color.
However, "No Color" is not a color.
Try:
color_mode="24-BIT_TRUE_COLOR",
background_color="255,0,255",
transparent_color="255,0,255"
255 0 255 is magenta (also called minus blue).
Of course, if you are using magenta in the map you may want to pick a different color
or rethink your symbol colors... please 😉
If you really need 8 bit color, then you need to pick one of your palleted colors to sacrifice to transparancy.