Yes, I just upgraded to ArcGIS 10 and installed the service pack and my image service will not start now with our .ecw aerial. You can't even pick it when you create a new service as I could in 9.3.
ESRI indicates that you must contact ERDAS to obtain a license to use with ArcGIS Server. I thought this would be like obtaining a license from UNISYS to use .gif (which is not public domain) but no - the charge is $3600 for an 8 core server. We will be using a different format from this point forward - particularly since our imagery ends up as a cache anyway.The whole reason the ECW file format was invented is so you do not need a cache to serve the image with inexpensive hardware and low bandwidth networks with high performance. Compression was a nice way to shrink the image data at the same time.