I am making layout files for specific types of maps for others to use. Some of these maps require a scale of 1:24,000. Is it possible to lock the scale of a map frame in a layout file so the required scale is already loaded when others use the layout files?
Absolutely! The Fixed Scale map frame constraint locks the scale for you. Learn more here https://pro.arcgis.com/en/pro-app/latest/help/layouts/customizing-your-map-extent.htm