Hi, Robert. Thanks for responding.
No, making a new copy of the APRX resulted in the same error when opening the copy. The APRX would not package throwing a heap of Layer data source is inaccessible errors.
This problem has happened to two APRX files this week. The first to fail was a base map that I do my daily work in, the second was a copied version of the first but with some data removed and renamed.
I have tried busting into the APRX with 7zip and deleting out old data from Layers folder but that had no impact.