We have ArcGIS Enterprise Portal 10.9.1 with HA environment configured with IWA.
I have also an esri case open for this issue. We have configured the app pool for the portal web adaptors according to the guidelines here How To: Configure Integrated Windows Authentication with a highly-available portal (esri.com)
We only have one external load balancer fronting the two web adaptor machines and the admin access through web adaptors are enabled true for that reason. The portals and federated arcgis servers have instances in the two web adaptor machines.
Everything seems to be working fine, except for when we disable the anonymous access to the portal web adaptor instances in the web adaptor machines, the Settings page under the Organization/Settings will not load at all. It seems like it is not able to communicate to the federated servers when anonymous is disabled.
Other tabs like Members, Licenses, Status, they load without any issue.
We have provided fiddler saz file, screen shot of error and logs to esri support already.
Thanks!