I installed ArcGIS Enterprise, Portal and server works fine but when I tried to access items from portal folder I receive a warning saying to sign in to access the item. Did I miss some config?
it looks like you're using a hostname in the 'web map':
ec2amaz-4m3apc1
you would typically using a fully-qualified domain name there:
ec2amaz-4m3apc1.domain.com
the fully qualified domain name is typically trusted whereas the hostname is not. Have you got an alias to use? That's a particularly gruesome URL otherwise. I strongly recommend create an alias before you start loading any content. e.g. mygis.domain.com.
Just to add to Scott's answer, if this is a new Enterprise and you've registered the web adaptor for Portal and Server - you may need to amend the web adaptors to use a friendly DNS alias on the registration page. Please see:
https://enterprise.arcgis.com/en/web-adaptor/latest/install/iis/configure-arcgis-web-adaptor-portal.htm#:~:text=the%20Web%20Adaptor%20should%20be%20configured%20over%20the%20DNS%20alias%20instead
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