Of these three, "layer order, or symbology, or definition query changing", only changing the layer definition is supported.Reference:http://help.arcgis.com/en/webapi/flex/apiref/com/esri/ags/layers/ArcGISDynamicMapServiceLayer.html#layerDefinitionsYou could possibly workaround layer order issues by using multiple ArcGISDynamicMapServiceLayers and controlling the visibleLayers in each one.
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