Jamal, this is absolutely possible to do in IIS. However, most of these settings are not exposed via the GUI. Please read up the documentation on IIS for finding out which way of authentication would work best for you. There are some guidelines on setting this type of security you are looking for (basically allow accessing a web application only to certain users of Active Directory), but I guess you might ask for help for your IT folks since it is not a trivial task.The link for blog post works fine for me. If when clicking the ArcGIS blog opens, just click on the link again and it should work. If you Google "arcgis silverlight security" the first result would be the link to this page too.
Jamal, consider updating to the 3.1 version which has functionality for challenging users with the username/password to access secured ArcGIS Server services you've added in the Builder. The pop-up window on the application load will let define what services will show on the map and what not (so if a web app consists of three map services while a certain user has access only to one of them, the application enters its username and password and then the web app will load yet the only one map service will be shown).PS. Just in case you would want to implement secured access on the web application level, consider reading this Esri blog post with detailed instructions on how to do this in IIS. In this case, you secure the whole web application and not services itself (so called Web Tier authentication).
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